The New York-based international rights group said it would be shameful if the United Nations failed to carry out a proper investigation into the alleged attacks.
The call is a follow-up to the alarm it raised in September last year, claiming it had credible evidence of the repeated use of chemical weapons, which killed more than two hundred people in the Darfur region earlier in the year.
Amnesty claims Sudan’s military carried out coordinated ground and air strikes, targeting civilians, between January and August last year.
The rights group says it used satellite imagery and eyewitness and victim accounts to gather its evidence.
But Sudan has denied the allegations, saying it did not own chemical weapons and had never used them.