A statement from the International Olympic Committee said re-analysis of team member Nesta Carter’s Beijing sample had tested positive for the prohibited substance methylhexaneamine.
The decision to strip Jamaica of their 4x100m title deprives Bolt of one of his nine golds won across three Games between 2008 and 2016.
Carter ran the first leg of the Beijing final as Jamaica set a new world record, a mark they surpassed in London four years later.
Trinidad and Tobago are now in line for promotion to the gold medal position, with Japan taking silver and Brazil bronze.
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